human immortal c28 Search Results


human chondrocyte cell line c28/i2  (Merck KGaA)
90
Merck KGaA human chondrocyte cell line c28/i2
Generalizability of the suppression of inflammatory stimuli in miR-766-3p-transfected cells. ( A ) NHMCs were transfected with miRNA mimics (30 nM) and exposed to TNF-α or IL-1β for 24 h. The expression of the indicated genes was examined by a qPCR. ( B , C ) <t>C28/I2</t> cells ( B ) and NHMCs ( C ) were co-transfected with pNF-κB-Luc along with miRNA mimics (C28/I2: 5 nM, NHMC: 30 nM). After incubation, cells were treated with TNF-α or IL-1β for 6 h and subjected to a luciferase assay to evaluate the activity of NF-κB. The luciferase activity was normalized by the number of viable cells and then normalized to the respective values in the vehicle samples (upper bar graphs). The lower scatterplots show the frequency of NF-κB inhibition by miR-766-3p. Assays were performed in quadruplicate. Data are expressed as the mean ± SEM. Asterisks indicate statistically significant differences ( p < 0.05).
Human Chondrocyte Cell Line C28/I2, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human chondrocyte cell line c28/i2/product/Merck KGaA
Average 90 stars, based on 1 article reviews
human chondrocyte cell line c28/i2 - by Bioz Stars, 2026-04
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human immortal c28/i2 cells  (Procell Inc)
90
Procell Inc human immortal c28/i2 cells
Generalizability of the suppression of inflammatory stimuli in miR-766-3p-transfected cells. ( A ) NHMCs were transfected with miRNA mimics (30 nM) and exposed to TNF-α or IL-1β for 24 h. The expression of the indicated genes was examined by a qPCR. ( B , C ) <t>C28/I2</t> cells ( B ) and NHMCs ( C ) were co-transfected with pNF-κB-Luc along with miRNA mimics (C28/I2: 5 nM, NHMC: 30 nM). After incubation, cells were treated with TNF-α or IL-1β for 6 h and subjected to a luciferase assay to evaluate the activity of NF-κB. The luciferase activity was normalized by the number of viable cells and then normalized to the respective values in the vehicle samples (upper bar graphs). The lower scatterplots show the frequency of NF-κB inhibition by miR-766-3p. Assays were performed in quadruplicate. Data are expressed as the mean ± SEM. Asterisks indicate statistically significant differences ( p < 0.05).
Human Immortal C28/I2 Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human immortal c28/i2 cells/product/Procell Inc
Average 90 stars, based on 1 article reviews
human immortal c28/i2 cells - by Bioz Stars, 2026-04
90/100 stars
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human immortalized c28/i2 cells  (Biochrom)
90
Biochrom human immortalized c28/i2 cells
Generalizability of the suppression of inflammatory stimuli in miR-766-3p-transfected cells. ( A ) NHMCs were transfected with miRNA mimics (30 nM) and exposed to TNF-α or IL-1β for 24 h. The expression of the indicated genes was examined by a qPCR. ( B , C ) <t>C28/I2</t> cells ( B ) and NHMCs ( C ) were co-transfected with pNF-κB-Luc along with miRNA mimics (C28/I2: 5 nM, NHMC: 30 nM). After incubation, cells were treated with TNF-α or IL-1β for 6 h and subjected to a luciferase assay to evaluate the activity of NF-κB. The luciferase activity was normalized by the number of viable cells and then normalized to the respective values in the vehicle samples (upper bar graphs). The lower scatterplots show the frequency of NF-κB inhibition by miR-766-3p. Assays were performed in quadruplicate. Data are expressed as the mean ± SEM. Asterisks indicate statistically significant differences ( p < 0.05).
Human Immortalized C28/I2 Cells, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human immortalized c28/i2 cells/product/Biochrom
Average 90 stars, based on 1 article reviews
human immortalized c28/i2 cells - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Generalizability of the suppression of inflammatory stimuli in miR-766-3p-transfected cells. ( A ) NHMCs were transfected with miRNA mimics (30 nM) and exposed to TNF-α or IL-1β for 24 h. The expression of the indicated genes was examined by a qPCR. ( B , C ) C28/I2 cells ( B ) and NHMCs ( C ) were co-transfected with pNF-κB-Luc along with miRNA mimics (C28/I2: 5 nM, NHMC: 30 nM). After incubation, cells were treated with TNF-α or IL-1β for 6 h and subjected to a luciferase assay to evaluate the activity of NF-κB. The luciferase activity was normalized by the number of viable cells and then normalized to the respective values in the vehicle samples (upper bar graphs). The lower scatterplots show the frequency of NF-κB inhibition by miR-766-3p. Assays were performed in quadruplicate. Data are expressed as the mean ± SEM. Asterisks indicate statistically significant differences ( p < 0.05).

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling

doi: 10.3390/ijms20040809

Figure Lengend Snippet: Generalizability of the suppression of inflammatory stimuli in miR-766-3p-transfected cells. ( A ) NHMCs were transfected with miRNA mimics (30 nM) and exposed to TNF-α or IL-1β for 24 h. The expression of the indicated genes was examined by a qPCR. ( B , C ) C28/I2 cells ( B ) and NHMCs ( C ) were co-transfected with pNF-κB-Luc along with miRNA mimics (C28/I2: 5 nM, NHMC: 30 nM). After incubation, cells were treated with TNF-α or IL-1β for 6 h and subjected to a luciferase assay to evaluate the activity of NF-κB. The luciferase activity was normalized by the number of viable cells and then normalized to the respective values in the vehicle samples (upper bar graphs). The lower scatterplots show the frequency of NF-κB inhibition by miR-766-3p. Assays were performed in quadruplicate. Data are expressed as the mean ± SEM. Asterisks indicate statistically significant differences ( p < 0.05).

Article Snippet: The human synovial fibroblast cell line MH7A was obtained from Riken Cell Bank (Ibaraki, Japan) [ ], the human chondrocyte cell line C28/I2 was obtained from Merck Millipore (Darmstadt, Germany), and normal human mesangial cells (NHMCs) were obtained from Lonza (Basel, Switzerland).

Techniques: Transfection, Expressing, Incubation, Luciferase, Activity Assay, Inhibition